A multidrug-resistant CTX-M-15-producing Klebsiella pneumoniae (KpP1 strain) was isolated from a native Amazonian fish (Brachyplatystoma filamentosum) at the Brazilian Amazon . The strain was identified by MALDI-TOF . The genome was extracted, purified and a Nextera DNA Flex library was prepared and sequenced by Illumina platform . The sequenced genome was de novo assembled using Unicycler and in silico prediction accomplished by curated bioinformatics tools . The size of the genome is 5.6 Mb with 5715 genes . Whole-genome sequencing analysis revealed the presence of wide resistome, with genes conferring resistance to clinically relevant antibiotics, heavy metals and disinfectants . The KpP1 strain was assigned to the sequence type ST3827, KL111 (wzi113) and O3b locus . Native freshwater fish sold in wet markets of the Amazonian region could be an important vehicle for transmission of multidrug-resistant bacteria to humans . This study may give genomic insights on the spread of critical-priority WHO pathogens in a One Health context.