Pesticide-induced DNA damage is primarily repaired by base excision repair (BER) pathway . However, polymorphism in DNA repair genes may modulate individual's DNA repair capacity (DRC) leading to increased genotoxicity and adverse health effects . Our first study in North-West Indian population aimed to evaluate the impact of rs1052133 (Ser326Cys; C1245G), rs1799782 (Arg194Trp; C26304T) and rs25487 (Arg399Gln; G28152A) polymorphisms on the modulation of pesticide-induced DNA damage in a total of 450 subjects (225 pesticide-exposed agricultural workers and 225 age- and sex-matched controls). DNA damage was estimated by alkaline comet assay using silver-staining method . Genotyping was carried out by PCR-RFLP using site-specific restriction enzymes . Mann-Whitney U-test revealed elevation in DNA damage parameters (< 0.01) in pesticide-exposed agricultural workers than controls . Chi-square test showed significant (< 0.05) differences in the Arg194Trp (C26304T) and Arg399Gln (G28152A) genotypes among two groups . Multivariate logistic-regression analysis revealed that heterozygous genotypes of rs1052133 (326Ser/Cys; 1245CA), rs1799782 (194Arg/Trp; 26304CT) and rs25487 (399Arg/Gln; 2815GA) were positively associated (< 0.05) with elevated DNA damage parameters in pesticide-exposed agricultural workers . Our results strongly indicate significant positive association of variant and genotypes with reduced DRC and higher pesticide-induced DNA damage in North-West Indian agricultural workers.