Anti-müllerian hormone (AMH) is an established marker of ovarian reserve that decreases with age . Though the pool of ovarian follicles is established during fetal development, impacts of in utero exposures on AMH are uncertain . Thus, we sought to evaluate associations of in utero exposures with AMH of adult daughters with a prospective cohort study of adult daughters at university medical centers . Women noted their mother's reported use of diethylstilbestrol (DES), vitamins, tobacco, alcohol, and caffeine during pregnancy, and their mother's occupation during pregnancy . All participants were reproductive age women (18-40 years) enrolled in the Effects of Aspirin in Gestation and Reproduction (EAGeR) trial . Serum AMH concentrations were measured at baseline prior to conception and categorized using clinical guidelines . Multinomial regression models estimated associations between each exposure and high (> 3.5 ng/mL) and low (< 1.0 ng/mL) versus normal AMH (1.0-3.5 ng/mL), adjusting for participant's age, mother's age, mother's history of fertility treatment, and mother's use of vitamins . In 1202 women with available data, maternal caffeine use was associated with an increased risk of low AMH, compared to normal (relative risk [RR] 1.90 , 95% confidence interval [CI] 1.09 , 3.30). Vitamins were associated with an increased risk of high AMH compared to normal (RR 1.93 , 95% CI 1.24 , 3.00). Other exposures were not associated with AMH concentrations in offspring . Maternal caffeine and vitamin use during pregnancy may be associated with ovarian reserve in adult offspring, highlighting the potential importance of pregnancy lifestyle on the reproductive health of daughters.