BACKGROUND Ultrasound is a safe, non-invasive and affordable imaging technique for the visualization of internal structures and the measurement of blood velocity using Doppler imaging . However, despite all these advantages, no study has identified the structures of the rat brain using conventional ultrasound .
METHODS A 13 MHz high frequency transducer was used to identify brain structures in the rat . The enlargement of the transcranial window was performed gradually using the ultrasound directly on the skin of the animal, then against the skull, then through a delimited craniotomy and finally through a complete craniotomy .
RESULTS Our results showed that ultrasound allowed the identification of cerebral ventricles and subarachnoid cisterns, as well as the analysis of real-time monitoring of cerebral blood flow in the main brain arteries of the rat . COMPARISON WITH EXISTING METHODS Ultrasound is a tool with the potential to identify brain structures and blood vessels . In contrast to MRI, transcranial ultrasound is a fast, non-invasive, well tolerated and low-cost method and can be done at the bedside .
CONCLUSION In the present study, we described an atlas of the main brain structures as well as the main vasculature in the rat using ultrasound . This technique could be applied in animal models of various neurological diseases.