Algal biofuels are a promising alternative to fossil fuels, but their widespread use is hindered by problems with mass production . Light-emitting diodes (LEDs) with specific light wavelengths could be used as an energy source for algal growth and lipid synthesis . In this study, the effects of light source on the biomass and lipid production of the aerial microalga Coccomyxa sp . KGU-D001 were evaluated using LEDs . The integration of two-phase cultures, including growth and lipid production under the stress of nitrate depletion, was assessed for efficient lipid production under liquid- or aerial-phase conditions . Different wavelengths of light (blue, green, and red) were tested under liquid- and aerial-phase conditions . Under aerial-phase culture, the fatty acid contents in biofilm reached 320 mg g DWC with the red LEDs . In view of these findings, we describe a one-step culture method for growth and lipid accumulation in algal biofilm under aerial-phase culture with red LED irradiation . When Coccomyxa biofilm was cultured on wet cotton wool with BBM in a petri dish under the red LED, it was able to grow and accumulate lipids under the aerial-phase condition . Based on the results of this study, a potential method for a continuous biodiesel production system is proposed.