The objective of the study was to assess the prevalence and factors associated with household (HH) handwashing practice in Bangladesh and draw a trend of COVID-19 spreads and compare that with the countrywide HH handwashing practice . The study is based on the two national representative publicly available datasets (MICS 2019, and confirmed cases of COVID-19). Of 61,209 (weighted) HH, the overall prevalence of HH handwashing was found 56.3%, and the prevalence was significantly varied across the socio-economic status of the HH . Map comparison suggested that the gradual increasing trend of COVID-19 cases in areas where HH handwashing practice is low . The northern part of Bangladesh had the highest handwashing practice, whereas it had less effected by COVID-19 cases . However, central Bangladesh had the hardest hit by COVID-19 cases, and it had around 50% handwashing practice coverage . Large-scale observational study is necessary to establish the causality.