Abnormal environmental conditions induce polyploidization and exacerbate vulnerability to agricultural production . Polyploidization is a pivotal event for plant adaption to stress and the expansion of transcription factors . NACs play key roles in plant stress resistance and growth and development, but the adaptive mechanism of NACs during plant polyploidization remain to be explored . Here, we identified and analyzed NACs from 15 species and found that the expansion of NACs was contributed by polyploidization . The regulatory networks were systematically analyzed based on polyomics . NACs might influence plant phenotypes and were correlated with amino acids acting as nitrogen source, indicating that NACs play a vital role in plant development . More importantly, in quinoa and Arabidopsis thaliana, NACs enabled plants to resist stress by regulating flavonoid pathways, and the universality was further confirmed by the Arabidopsis population . Our study provides a cornerstone for future research into improvement of important agronomic traits by transcription factors in a changing global environment.