The clinical false negative rate of reverse transcriptase polymerase chain reaction (RT-PCR) testing for SARS-CoV-2 on a single upper respiratory tract sample was calculated using convalescent antibody testing as a comparator . The sensitivity in symptomatic individuals was 86.2% (25/29). Of the missed cases, one (3.5 %) was detected by repeat RT-PCR, one by CT thorax and two (7.1 %) by convalescent antibody . The clinical false negative rate of a single RT-PCR on an upper respiratory tract sample of 14% in symptomatic patients is reassuring when compared to early reports . This report supports a strategy of combining repeat swabbing, use of acute and convalescent antibody testing and CT thorax for COVID-19 diagnosis.