Globally, little evidence exists on transmission patterns of COVID-19 . Recommendations to prevent infection include appropriate and frequent handwashing plus physical and social distancing . We conducted an exploratory observational study to assess compliance with these recommendations in selected transportation stations in Ghana . A one-hour audit of 45 public transport stations in the Greater Accra region was carried out between 27th and 29th March 2020 . Using an adapted World Health Organization (WHO) hand hygiene assessment scale, the availability and use of handwashing facilities, social distancing, and ongoing public education on COVID-19 prevention measures were assessed, weighted and scored to determine the level of compliance of stations . Compliance with recommendations was categorized as``inadequate"`` basic",``intermediate"and``advanced", based on the overall score . Majority (80 %) of stations in Accra have at least one Veronica Bucket with flowing water and soap, but the number of washing places at each station is not adequate . Only a small minority (18 %) of stations were communicating the need to wash hands frequently and appropriately, and to practice social/physical distancing while at the station . In most stations (95 %), hand washing practice was either not observed, or only infrequently . Almost all stations (93 %) did not have alcohol-based hand sanitizers available for public use, while social distancing was rarely practiced (only 2 %). In over 90% of the stations, face masks were either not worn or only worn by a few passengers . Compliance with COVID-19 prevention measures was inadequate in 13 stations, basic in 16 stations, intermediate in 7 stations, and advanced in 9 stations . Compliance with COVID-19 prevention measures in public transportation stations in the Greater Accra region remains a challenge . Awareness creation should aim to elevate COVID-19 risk perception of transportation operators and clients . Transport operators and stations need support and guidance to enforce hand washing and social distancing.