The aim of present study is to investigate whether Ferulic acid (FA), a natural polyphenol antioxidant, was able to protect ARPE-19 cells from hydrogen peroxide (H2 O2) -induced damage, and elucidate the underlying mechanisms . Our results revealed that FA pre-treatment for 24 hours can reverse cell loss of H2 O2 -induced ARPE-19 cells via the promotion of cell proliferation and prevention of apoptosis, as evidenced by 5-ethynyl-2'-deoxyuridine (EdU) incorporation and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labelling (TUNEL) assay, respectively . Moreover, the addition of FA (5 mM) can decrease Bax and cleaved caspase-3 protein expression, but increase Bcl-2 protein expression in ARPE-19 cells . Furthermore, H2 O2 -induced oxidative stress in ARPE-19 cells was significantly alleviated by FA, illustrated by reduced levels of ROS and MDA . In addition, the attenuated antioxidant enzymes activities of (SOD, CAT and GPX) and GSH level were reversed almost to the normal base level by the pre-addition of FA for 24 hours . In all assays, FA itself did not exert any effect on the change of the above parameters . These novel findings indicated that FA effectively protected human ARPE-19 cells from H2 O2 -induced oxidative damage through its pro-proliferation, anti-apoptosis and antioxidant activity, suggesting that FA has a therapeutic potential in the prevention and treatment of AMD.