In the last few decades, there has essentially been an explosion in the use of lasers in medicine, especially in the area of cosmetic dermatology . Potentially harmful substances are liberated when tissues are vaporized with laser . This creates numerous risks, including the spread of infectious disease . Smoke evacuators are devices that capture and filter laser plume, thereby maintaining a safe environment for the surgical team and patient . Our aim was to characterize the microbial community structure within the suction tube and funnel of the smoke evacuator system, identify their origin, and evaluate pathogenicity . Dust particles were collected from the instruments with a cotton swab . DNA was extracted from the swabs and the transport media, and sequencing was performed using the Illumina HiSeq Xplatform . Metagenomic analysis was conducted using the Empowering the Development of Genomics Expertise (EDGE) Bioinformatics pipeline and custom Python scripts . The most abundant bacterial species were Micrococcus luteus and Brevibacterium casei in the suction tube, and Dermacoccus sp . Ellin 185 and Janibacter hoylei in the suction funnel . A total of 15 medium- to high-quality metagenome-assembled genomes (MAGs) were constructed where we found 104 antibiotic-resistant genes (ARGs) and 741 virulence factors . Findings indicate that the suction tube and funnel are likely a reservoir of virulence factor genes and ARGs, which can possibly be passed on to other bacteria via horizontal gene transfer . We would like to emphasize the health risk these microorganisms pose and the need to reevaluate the current hygiene standards with regard to the smoke evacuator system.