Background Coronavirus Disease 2019 (COVID-19) has caused a severe outbreak and become a global public health priority . Rapid increment of infection number along with significant deaths have placed the virus as a serious threat to human health . Rapid, reliable, and simple diagnostic methods are critically essential for disease control . While Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) is the current diagnostic gold standard, Reverse Transcriptase LoopMediated Isothermal Amplification (RT-LAMP) appears as a compelling alternative diagnostic test due to its more simplicity, shorter time to result, and lower cost . This study examined RT-LAMP application for rapid identification of SARS-CoV-2 infection compared to RT-PCR assay . Methods A systematic review and meta-analysis (2020) was conducted in 6 scientific databases following the PRISMA Guideline . Original published studies on human clinical samples in English were included . Articles evaluated sensitivity and specificity of RT-LAMP relative to RT-PCR were considered eligible . Quality assessment of bias and applicability was examined based on QUADAS-2 . Results A total of 351 studies were found based on the keywords and search queries . 14 eligible case control studies fitted the respective criteria . Quality assessment using QUADAS-2 indicated low risk bias in all included studies . All case studies, comprises 2,112 samples, had the cumulative sensitivity of 95.5% (CI 97.5% =90.8-97.9 %) and cumulative specificity of 99.5% (CI 97.5% =97.7-99.9 %). Conclusion RT-LAMP assay could be suggested as a reliable alternative COVID-19 diagnostic method with reduced cost and time compared to RT-PCR . RT-LAMP could potentially be utilized during the high-throughput and high-demand critical situations.