Background The COVID-19 pandemic had many implications on healthcare services, including cellular pathology . The pandemic-related lockdown was applied in Jordan from March to May 2020 . King Abdullah University Hospital (KAUH) was chosen to provide care for COVID-19 patients during that period . Since there was no experience in dealing with COVID-19 patients, the hospital maintained some essential services but canceled elective surgeries and procedures . The rationale was to prioritize care for COVID-19 patients and to provide better adherence to infection control policies and protect non-infected patients and healthcare workers . The purpose of the present study is to investigate the impact of COVID-19 pandemic restrictions on cellular pathology practice patterns at KAUH . Methods This is a retrospective observational study conducted at KAUH . All cellular pathology reports during the 2020 national lockdown were retrieved . The total numbers of specimens including types and procedures were recorded . Data were compared with the corresponding data in 2019 when there was no pandemic and when hospital and laboratory services were run in full capacity . Results 2020 lockdown period showed a 57.9% reduction in the total number of specimens received at the cellular pathology laboratory as compared to the corresponding period of 2019 (1400 versus 3322). Emergency procedures have represented 99.1% of the service during the lockdown with a remarkable diversity shift . Conclusion There was a significant drop in the number of specimens dealt with at KAUH cellular pathology laboratory during the COVID-19 pandemic-related national lockdown . We learned from this pandemic how to adapt to such circumstances by adjusting our way of working to reach the best level of staff safety while maintaining highly productive work . Implementing digital pathology platforms, working from home strategies and alternative training methodologies have emerged as an essential need.