Web-based experimental testing has seen an exponential growth in psychology and cognitive neuroscience . However, paradigms involving affective auditory stimuli have yet to adapt to the online approach due to concerns about the lack of experimental control and other technical challenges . In this study, we assessed if sounds commonly used to evoke affective responses in-lab can be used online . Using recent developments to increase sound presentation quality, we selected 15 commonly used sound stimuli and assessed their impact on valence and arousal states in a web-based experiment . Our results reveal good test-retest reliability and good internal consistency, with results comparable to in-lab studies . Additionally, we compared a variety of previously used unpleasant stimuli, allowing us to identify the most aversive of these sounds . Our findings demonstrate that affective sounds can be reliably delivered through web-based platforms, which helps facilitate the development of new auditory paradigms for affective online experiments.