Introduction The present analysis aims to evaluate the consequences of a 2-month interruption of mammographic screening on breast cancer (BC) stage at diagnosis and upfront treatments in a region of Northern Italy highly affected by the severe acute respiratory syndrome-related coronavirus-2 (SARS-CoV-2) virus . Methods This retrospective single-institution analysis compared the clinical pathological characteristics of BC diagnosed between May 2020 and July 2020, after a 2-month screening interruption, with BC diagnosed in the same trimester of 2019 when mammographic screening was regularly carried out . Results The 2-month stop in mammographic screening produced a significant decrease in in situ BC diagnosis (−10.4 %) and an increase in node-positive (+11.2 %) and stage III BC (+10.3 %). A major impact was on the subgroup of patients with BC at high proliferation rates . Among these, the rate of node-positive BC increased by 18.5% and stage III by 11.4% . In the subgroup of patients with low proliferation rates, a 9.3% increase in stage III tumors was observed, although node-positive tumors remained stable . Despite screening interruption, procedures to establish a definitive diagnosis and treatment start were subsequently carried out without delay . Conclusion Our data showed an increase in node-positive and stage III BC after a 2-month stop in BC screening . These findings support recommendations for a quick restoration of BC screening at full capacity, with adequate prioritization strategies to mitigate harm and meet infection prevention requirements.