As the coronavirus disease 2019 pandemic keep tackling global public health systems worldwide . The severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) genome keeps mutating . In that regard, the recent emergence of the B.1.1.7 lineage in the UK has called the attention of global authorities . One point of concern is that if this lineage can be detected by traditional molecular schemes for SARS-CoV-2 detection . Herein, we showed that this lineage does not affect the Berlin-Charité protocol but can challenge the available commercial kits directed to the Spike (S) gene . All efforts should be made to continue to monitor SARS-CoV-2 genomes for potential variants that can impair diagnostic testing and lead to false negative results.