The COVID-19 pandemic, caused by the SARS-CoV-2 infection, is a global health crisis . While many patients have clinically recovered, little is known about long-term alterations in T cell responses of COVID-19 convalescents . In this study, T cell responses in peripheral blood mononuclear cells of a long-time COVID-19 clinically recovered (20-26 weeks) cohort (LCR) were measured via flow cytometry and ELISpot . The T cell responses of LCR were comparatively analyzed against an age and sex matched short-time clinically recovered (4-9 weeks) cohort (SCR) and a healthy donor cohort (HD). All volunteers were recruited from Wuhan Jinyintan Hospital, China . Phenotypic analysis showed that activation marker PD-1 expressing on CD4+ T cells of LCR was still significantly lower than that of HD . Functional analysis indicated that frequencies of Tc2, Th2 and Th17 in LCR were comparable to those of HD, but Tc17 was higher than that of HD . In LCR, compared to the HD, there were fewer IFN-Î³ producing T cells but more IL-2 secreting T cells . In addition, the circulating Tfh cells in LCR were still slightly lower compared to HD, though the subsets composition had recovered . Remarkably, SARS-CoV-2 specific T cell responses in LCR were comparable to that of SCR . Collectively, T cell responses experienced long-term alterations in phenotype and functional potential of LCR cohort . However, after clinical recovery, SARS-CoV-2 specific T cell responses could be sustained at least for six months, which may be helpful in resisting re-infection.