More than 65 million people have been confirmed infection with SARS-CoV-2 and more than 1 million have died from COVID-19 and this pandemic remains critical worldwide . Effective vaccines are one of the most important strategies to limit the pandemic . Here, we report a construction strategy of DNA vaccine candidates expressing full length wild type SARS-CoV-2 spike (S) protein, S1 or S2 region and their immunogenicity in mice . All DNA vaccine constructs of pCMVkan-S, -S1 and -S2 induced high levels of specific binding IgG that showed a balance of IgG1/IgG2a response . However, only the sera from mice vaccinated with pCMKkan-S or -S1 DNA vaccines could inhibit viral RBD and ACE2 interaction . The highest neutralizing antibody (NAb) titer was found in pCMVkan-S group, followed by -S1, while -S2 showed the lowest PRNT50 titers . The geometric mean titers (GMTs) were 2,551 , 1,005 and 291 for pCMVkan-S, -S1 and -S2, respectively . pCMVkan-S construct vaccine also induced the highest magnitude and breadth of T cells response . Analysis of IFN-γ positive cells after stimulation with SARS-CoV-2 spike peptide pools were 2,991 , 1,376 and 1,885 SFC/106 splenocytes for pCMVkan-S, -S1 and -S2, respectively . Our findings highlighted that full-length S antigen is more potent than the truncated spike (S1 or S2) in inducing of neutralizing antibody and robust T cell responses.