The spike (S) protein mutations of SARS-CoV-2 are of major concern in terms of viral transmission and pathogenesis . Hence, we developed a PCR-based method to rapidly detect the 6 mutational hotspots (H49Y, G476S, V483A, H519Q, A520S, and D614G) in the S protein and applied this method to analyze the hotspots in the viral isolates from different geographical origins . Here, we identified that there was only the D614G mutation in the viral isolates . As of September 30 , 2020, the analysis of 113,381 sequences available from the public repositories revealed that the SARS-CoV-2 variant carrying G614 has become the most prevalent form globally . Our results support recent epidemiological and genomic data demonstrating that the viral infectivity and transmission are enhanced by the S protein D614G mutation.
Index: ACE2, angiotensin-converting enzyme-2, COVID-19, Coronavirus disease, CT, cycle threshold, D614G mutation, Different geographic origins, E, envelope, M, membrane, Mutational hotspots, N, nucleocapsid, NGS, next-generation sequencing, Nsp3, nonstructural protein, Orf, open reading frame, RDB, receptor-binding domain, RT-qPCR, reverse transcriptase-quantitative polymerase chain reaction, RdRp, RNA-dependent RNA polymerase, S, Spike, SARS-CoV-2, SARS-CoV-2, severe acute respiratory syndrome coronavirus 2, Spike gene, Spike protein, TMPRSS2, transmembrane serine protease2