Toscana virus (TOSV) can cause central nervous system infections in both residents of and travelers to Mediterranean countries . Data mining identified three real-time RT-qPCR assays for detecting TOSV RNA targeting non-overlapping regions in the nucleoprotein gene . Here, they were combined to create a multi-region assay named Trio TOSV RT-qPCR consisting of six primers and three probes . In this study, (i) we evaluated in silico the three RT-qPCR assays available in the literature for TOSV detection, (ii) we combined the three systems to create the Trio TOSV RT-qPCR, (iii) we assessed the specificity and sensitivity of the three monoplex assays versus the Trio TOSV RT-qPCR assay, and (iv) we compared the performance of the Trio TOSV RT-qPCR assay with one of the reference monoplex assays on clinical samples . In conclusion, the Trio TOSV RT-qPCR assay performs equally or better than the three monoplex assays; therefore, it provides a robust assay that can be used for both research and diagnostic purposes.
Index: Mediterranean, Phenuiviridae, Phlebotomus, Phlebovirus, arbovirus, diagnostics, meningitis, sand fly