Background The coronavirus disease 2019 (COVID-19) pandemic is a major public health concern . Accurate and rapid diagnosis of COVID-19 is critical for disease control . Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a nucleic acid amplification assay similar to reverse transcription-polymerase chain reaction (RT-PCR), the former being a simple, low cost, and rapid method . Objectives This study aimed to compare the RT-LAMP assay with RT-PCR using the LoopampTM SARS-CoV-2 Detection Kit . Study Design One hundred and fifty-one nasopharyngeal swab and 88 sputum samples obtained from individuals with suspected or confirmed COVID-19 were examined . Results RT-LAMP had high specificity (98.5% (95% CI : 96.9–100 %) ), sensitivity (87.0% (95% CI : 82.8–91.3 %) ), positive predictive value (97.9% (95% CI : 96.1–99.7 %) ), negative predictive value (90.2% (95% CI : 86.4–94.0 %) ), and concordance rate (93.3% (95% CI : 90.1–96.5 %) ). Nasopharyngeal and sputum samples positive in RT-LAMP contained as few as 10.2 and 23.4 copies per 10 μL, respectively . RT-LAMP showed similar performance to RT-PCR for samples with cycle threshold value below 36 . Conclusions These results indicate that RT-LAMP is a highly reliable and at least equivalent to RT-PCR in utility, and potentially applicable in settings that are more diverse as a point-of-care tool.