A serological survey of human coronavirus antibodies among villagers in 10 provinces of Thailand was conducted during 2016-2018 . Serum samples (n = 364) were collected from participants from the villages and tested for coronavirus antibodies using a human coronavirus IgG ELISA kit . Our results showed that 10.44% (38/364; 21 males and 17 females) of the villagers had antibodies against human coronaviruses . The odds ratio for coronavirus positivity in the villagers in the central region who were exposed to bats was 4.75 , 95% CI 1.04-21.70, when compared to that in the non-exposed villagers . The sociodemographics, knowledge, attitudes and practices (KAP) of the villagers were also recorded and analysed by using a quantitative structured questionnaire . Our results showed that 62.36% (227/364) of the villagers had been exposed to bats at least once in the past six months . Low monthly family income was statistically significant in increasing the risk for coronavirus seropositivity among the villagers (OR 2.91 , 95% CI 1.13-7.49). In-depth interviews among the coronavirus-positive participants (n = 30) showed that cultural context, local norms and beliefs could influence to bat exposure activities . In conclusion, our results provide baseline information on human coronavirus antibodies and KAP regarding to bat exposure among villagers in Thailand.