Protection of healthcare workers (HCWs) against influenza is essential for patient health and a functional health system . The study aimed to analyze the demand for seasonal influenza vaccination (SIV) among various groups of HCWs in a tertiary care hospital before and during the COVID-19 pandemic and to identify their motives for this season's SIV . Before this influenza season (2020/21), the hospital management offered free SIV to all HCWs and promoted it on the internal network . Out of 4,167 HCWs , 630 HCWs expressed interest in SIV and were vaccinated in the hospital . They filled in a total of 603 self-administered pen-and-paper questionnaires . The mean age of the respondents (374 females and 229 males) was 45 ± 12 years . Physicians accounted for 48% of the vaccinated persons but for only 24% (p <.001) of all HCWs to whom SIV was offered . Only 16% of respondents vaccinated this year also received SIV before the last season (2019/20), with the proportion of physicians (19 %) being statistically significantly higher than that of non-physicians (13%, p = .045) and the proportion of chronically ill HCWs (22 %) being higher than that of healthy individuals (13%, p = .004). Most frequently, respondents' motivation to get vaccinated this year was self-protection (61 %), that is concerns about contracting influenza together with COVID-19 or alone, followed by family protection (58 %) and patient protection (53 %). In conclusion, COVID-19 contributed to an increased demand for SIV among HCWs and the threat of contracting it together with influenza was the most frequent motive.