Health care personnel (HCP) are at high risk for coronavirus disease-2019 acquisition . Serum antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) indicate past infection . Our institution offered SARS-CoV-2 antibody testing to HCP . We surveyed HCP with positive test results to explore past exposure to SARS-CoV-2, details of symptoms during the preceding 6 months, and a history of SARS-CoV-2 polymerase chain reaction testing . A total of 2162 HCP underwent antibody testing . Eight hundred fifty-seven (39.6 %) employees tested positive and, of these , 820 (95.7 %) participated in the survey . When adjusted for age, males had higher odds of testing positive for SARS-CoV-2 antibodies compared with females (OR = 1.68; 95% CI = 1.37-2.05; P = .00) and clinical staff had higher odds of SARS-CoV-2 seropositivity compared with nonclinical staff (OR = 1.273; 95% CI = 1.06-1.53; P = .01). Implementation of effective infection control measures is essential to protect HCP from coronavirus disease-2019.