Objectives Previous data indicate that children might play a less crucial role in SARS-CoV-2 transmission than initially assumed . We conducted a study to gain further knowledge on prevalence, transmission and spread of SARS-CoV-2 among preschool children, their parents and caretakers . Study design Children, their parents and care givers in 14 childcare facilities in Dresden, Saxony/ Germany were invited to participate in the KiTaCoviDD19-study between July 2020 and January 2021 . Seroprevalence of SARS-CoV-2 antibodies was assessed up to 4 times during the study period in all participating adults and personal characteristics as well as epidemiological information of personal SARS-CoV-2 history were obtained . Stool viral shedding of SARS-CoV-2 was analyzed every 2-4 weeks in all participating children . Results 318 children , 299 parents and 233 childcare workers were enrolled . By January 2021 11% of the participating adults were detected seropositive, whereas the rate of SARS-CoV-2 positive children was 6.8% . Overall, we detected 17 children with SARS-Cov-2 viral shedding in 8 different childcare facilities . In 4 institutions there occurred a maximum of three connected cases in children . About 50% of SARS-CoV-2 infections in children could not be connected to a secondary case within our study population . Conclusion This study could not provide evidence for a relevant asymptomatic (silent) spread of SARS-CoV-2 in childcare facilities, neither in a low nor a high prevalence setting . This finding adds to the evidence that childcare and educational settings do not play a crucial role in driving the SARS-CoV-2 pandemic.